Do you have PowerPoint slides to share? - Title: Genetic Engineering and Biotechnology Author: mpavicic Last modified by: mpavicic Created Date: 4/16/2010 5:45:23 PM Document presentation format, | PowerPoint PPT presentation | free to view. of E. coli) Screen for inability to acquire a selectable marker Proteins involved in DNA recombination (the E. coli paradigm) Mutation Phenotype RecA RecBC RecD RecF RecJ RecO RecR RecQ RecN RecG RuvA RuvB RuvC PriA PriB PriC DnaT Recombination deficient Reduced recombination Rec+  independent Reduced plasmid recombination • Reduced recombination in RecBC- • as above • as above • as above Reduced recombination in RecBC- Reduced recombination in RuvA-B-C- Reduced recombination in RecG- as above as above Reduced recombination as above as above as above, Chi (Χ) site: • 8 base pair sequence without • symmetry (5’GCTGGTCC) • Greatly stimulates ability of RecBCD • to catalyze recombination RecBCD exonuclease: opens strands • RecBCD binds to DNA at one end or at • a ds breakage point • Moves along the DNA, creating a loop • and degrading the strand with a free • 3’ end via its exonuclease activity • Exonuclease activity is inhibited • upon passing a Chi site of orientation • Upon cessation of exonuclease activity, • the undegraded 3’ end pairs with • homologous sequences on another • DNA molecule, RecA: needed to form triple helix • RecA binds to free strand to form an • extended helical structure. 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Genetic recombination occurs when genetic material is exchanged between two different chromosomes or between different regions within the same chromosome.We can observe it in both eukaryotes (like animals and plants) and prokaryotes (like archaea and bacteria).Keep in mind that in most cases, in order for an exchange to occur, the … ... PowerPoint Presentation - Beautifully designed chart and diagram s for PowerPoint with visually stunning graphics and animation effects. Variable region gene rearrangements ( VJ & V(D)J recombination). INTRODUCTION TO RECOMBINANT DNA TECHNOLOGY : INTRODUCTION TO RECOMBINANT DNA TECHNOLOGY BY Mrs. PRATHEEKSHA PILLAI PowerPoint Presentation : Definition All the techniques invovled in the construction, study and use of rDNA molecules. Bacteria Genetics Introduction Chromosome (bacteria are haploid; in other words, they have a single chromosome) Mutations (change in the base sequence of DNA) Extrachromosomal genetic material Plasmid Transposons Genetic recombination : Transfer of DNA from one bacterium to another Introduction Changes in the genome can occur by two processes Mutations Genetic recombination … People can be tested to diagnose if they have a certain disease and ... - Title: Genetic Engineering Author: Michael Last modified by: jtowner Created Date: 4/7/2008 11:51:22 PM Document presentation format: On-screen Show. Bacterial Recombination• Genetic recombination refers to the exchange of genes between twoDNA molecules to form new combinations of genes on achromosome.• To view this presentation, you'll need to allow Flash. PowerPoint Presentation : The genetic code is the set of rules by which information encoded within genetic material ( DNA or mRNA sequences) is translated into proteins by living cells . 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For animation, see http://www.sdsc.edu/journals/mbb/ruva.html, AmpR EmR b’ a a’ b Or b’ EmR a a a a 5’ end of gene a a Single cross-over results in one truncated copy and one intact copy of the gene a a internal fragment Single cross-over results in an interrupted gene a b a’ AmpR, a a a a Single cross-over outcome when using one end of the gene a a b P2 P1 a a b b P1 a a P2 Useful for introducing a promoter-reporter gene fusion without disrupting the gene’s function. Genetic recombination refers to the rearrangement of DNA sequences by the breakage and rejoining of chromosomes or chromosome segments. The process was first pos­tulated by […] (Nature 374, 375 (1995)). 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Site-specific recombinases (SSRs) perform rearrangements of DNA segments by recognizing and binding to short DNA sequences (sites). The PowerPoint PPT presentation: "Genetic Recombination and Genetic Engineering" is the property of its rightful owner. overview. 1967. meiotic gene conversion in yeast tetrads and the theory of, Hadronization of a QGP via recombination - Kang seog lee. Recombination and gene linkage paprescott. Boasting an impressive range of designs, they will support your presentations with inspiring background photos or videos that support your themes, set the right mood, enhance your credibility and inspire your audiences. Create Presentation Download Presentation. Multigene organisation of Ig genes 2. Definitions. Tags : Genetic Published on: Aug 18, 2018 Views: 1564 | Downloads: 2 . ?????????????" Recombination plays an important role in this process by allowing genes to be reassorted into different combinations. 班级： 生物学基地班 姓名： 陈浩 学号： 200431060028. what is the function of the homologous, Gene Concept - . chromosomes. Genetic maps: (linkage map) approximate locations of genes, relative to the location of other genes, based on the rates of recombination * * * * * Figure 20.1 Genetic maps are based on rates of recombination. Presentation Summary : Genetic Recombination Exchange of genes between two DNA molecules Forms new combination of genes on a chromosome Along with mutations, contributes to genetic. Get powerful tools for managing your contents. If so, share your PPT presentation slides online with PowerShow.com. What do get when you cross a bull dog ... Ch. DNA BREAKS ARE COMMON AND INITIATE RECOMBINATION. Latest developments. See more ideas about powerpoint templates, presentation templates, powerpoint. View Recombination+II+_NHEJ_,+WILSON+LECTURE+3.ppt from BIO 541 at University of Washington. PowerPoint Presentation : Vector DNA … DNA RECOMBINATION They are all artistically enhanced with visually stunning color, shadow and lighting effects. PowerPoint presentation | free to view - id: 4bd76e-MWVmO, The Adobe Flash plugin is needed to view this content. The detection of recombination in diploid organisms. • Happens during: • Independent assortment Genetic Recombination: The sorting of alleles into new combinations. Figure not in 3rd edition. Linkage and Recombination - University of Arizona PPT. Presentation Title: Genetic Recombination In Bacteria. nonrandom recombination events: Welcome! Many of them are also animated. Download Now. Genetic Recombination Author: Cynthia Last modified by: Cynthia Created Date: 2/29/2008 9:22:30 PM Document presentation format: On-screen Show Company: The … 10.3 of textbook, Models of Homologous Recombination (III) Double strand break-repair • A double stranded break occurs in one • molecule; and exonuclease digests the • 5’ ends of each break, leaving a gap • One of the 3’ tails invades unbroken • molecule; pairs with complementary • sequence • DNA polymerase extends the tail until • it can be joined with 5’ end • Displaced strand used as template to • replace gap on other molecule • Two Holliday junctions form (may • produce recombinant flanking DNA • depending how they are resolved) Fig. 09 lecture presentation lvalera. chonnam national, Genetic Mapping - . UV light) • detectable as reduced • recombination in RecBC- • background • Important after heteroduplex • formation is initiated • branch migration • resolution of heterduplex Proteins involved in DNA recombination (the E. coli paradigm) (con’t) Mutation Phenotype RecA RecBC RecD RecF RecJ RecO RecR RecQ RecN RecG RuvA RuvB RuvC PriA PriB PriC DnaT Recombination deficient Reduced recombination Rec+  independent Reduced plasmid recombination • Reduced recombination in RecBC- • as above • as above • as above Reduced recombination in RecBC- Reduced recombination in RuvA-B-C- Reduced recombination in RecG- as above as above Reduced recombination as above as above as above, RuvB RuvA RuvC resolves (cuts) the Holliday junction • Ruv C is a specialized endonuclease • an X-phile – cuts crossed DNA strands • always cuts at two T’s Efficient branch migration requires RuvA and RuvB • RuvA specifically binds Holliday junctions • - resultant structure better able to undergo branch migration and resolution • RuvB is a helicase • - forms a hexameric ring around the DNA strand • - DNA is pumped through the ring using ATP cleavage to drive the pump • - the synapse is thus forced to migrate, RuvA RuvB RuvB A simple model of a RuvA/RuvB/DNA complex extrapolating from the above model and in agreement with the electron microscopy results of Parsons et al. 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